{"id":1472,"date":"2020-11-13T15:25:17","date_gmt":"2020-11-13T15:25:17","guid":{"rendered":"http:\/\/cimacanarias.com\/?page_id=1472"},"modified":"2022-05-17T10:16:46","modified_gmt":"2022-05-17T10:16:46","slug":"things-we-do-in-cima-14-microbiological-analysis","status":"publish","type":"page","link":"https:\/\/cimacanarias.com\/en\/things-we-do-in-cima-14-microbiological-analysis\/","title":{"rendered":"Things we do in CIMA 14: Microbiological analysis"},"content":{"rendered":"

\"\"<\/a>From CIMA (Centro de Investigaciones Medioambientales del Atl\u00e1ntico, S.L.) (Atlantic Environmental Research Center), we want to show you what we do in and out our offices.<\/p>\n

On this page you can find a series of small videos called \u00abThings we do in CIMA\u00bb, which will help us to explain what we do and how we do it. All this to approach the world of marine biology and oceanography.<\/p>\n

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– \u00abThings we do in CIMA\u00bb #14: Microbiological analysis:<\/strong><\/p>\n

As you have seen in our flowmeter video<\/strong><\/a>, some of our services are closely linked to wastewater in general. Flow measurements, pollution levels or checking wastewater emissions legality are some of these services.<\/p>\n

One of our tasks is collecting samples in different points of the sewage system to analyze several parameters. Some of which are our microbiological analysis, with which we can know tipped water quality.<\/p>\n

Among other parameters, we usually measure health harmful bacteria<\/strong><\/a> presence and concentration, such as coliform<\/strong><\/a> and enterococcus<\/strong><\/a>.<\/p>\n

Facilitating these measures we have a Quanti-Tray Bag<\/strong><\/a> that helps us to count coliform most probable number (MPN)<\/strong><\/a>. We use the \u00abSingle dilution method\u00bb, which is a specifically designed test for coliform bacteria MPN counting (indicators of faecal contamination) from water samples and it is applicable to all human consumption water types.<\/p>\n

Colilert-18 technique<\/strong><\/a>. is based on the Defined Substrate Technology (DST) patented by IDEXX<\/strong><\/a>, which allows total coliforms and E. coli<\/strong><\/a> simultaneous detection. DST method uses indicator nutrients, that is reagents that cause color changes in water samples under study:<\/p>\n

– Total coliform: these bacteria metabolize<\/strong><\/a> bacteria chromogenic substrate<\/strong><\/a> and cause a staining change from transparent to yellow.<\/p>\n

– Escherichia coli: these bacteria also have the ability to metabolize substrates and, in addition, specifically metabolize a fluorogenic substrate<\/strong><\/a>, responsible for producing fluorescence<\/strong><\/a>.<\/p>\n

Thanks to these cells with reagents we can see total coliform presence and, on the other hand, we can differentiate cells with E. Coli, which is illuminated under ultraviolet light. We also use the same type of cells, with other reagents, for intestinal enterococcus detection.<\/p>\n

If concentrations exceed healthy limits, we warn responsible persons so that they act quickly.<\/p>\n

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Download to share by WhatsApp<\/strong><\/a> (4.6Mb).Remember, to download the video from the phone, click the link. If you are asked, choose to open the video with the browser and, once opened, keep your finger on it until you get a poster of this style: \u00abSave Video\u00bb. From now on, you only have to share, share and share!<\/p>\n

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